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1 Introduction In the 1930s, Nobel Prize-winning chemist Vincent du Vigneaud isolated homocysteine (Hcy) from bladder stones

This may produce severe adverse effects or even death

The strong association between high NQO1 expression and cancer progression has been supported by results of a study showing that the overexpression of NQO1 significantly induced cell cycle progression via the up regulation of cyclin A2, B1 and D1 which led to the melanoma cells proliferation [28]

doi: 10.1111/j.1567-1364.2005.00015.x 3 AlbergariaH.ArneborgN

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To purify His 6 -SUMO-tagged ArqI, the cell pellet was resuspended in 25 mL/L culture lysis/wash buffer (50 mM Tris-HCl pH 8.0 150 mM NaCl, 50 mM imidazole, 1 mM beta-mercaptoethanol (ME), and 1 mM phenylmethylsulfonyl fluoride (PMSF)) and sonicated for 5 x 30 s pulses using a Branson Sonifier S-450 sonicator